PMID:12997228

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Citation

LESTER, G (1952) The beta-galactosidase of lactose mutants of Escherichia coli, K-12.Arch. Biochem. Biophys. 40:390-401

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Escherichia coli; Glycoside Hydrolases

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:562

Y-53

  • Abolished utilization of carbon source (lactose)
  • Growth phenotype on EMB (grows on EMB, but has negative result on indicator for lactose)
  • Abolished lactose catabolic process

Growth

  • cannot utilize lactose
  • deficient in lactose metabolism

Plating Assay

plated on eosin-methylene blue agar

Escherichia coli

NCBI:562

Y-53

  • Utilization of carbon source (melibiose)
  • Growth on EMB (positive result on indicator)
  • Melibiose catabolic process
  • Increased rate of?

Growth

The authors compared the ability of different strains to ferment different sugars

  • ferment melibiose rapidly compared to control

Plating Assay

plated on eosin-methylene blue agar with 1% melibiose

Escherichia coli

NCBI:562

Y-53

  • Utilization of carbon source (xylose)
  • Xylose catabolic process
  • Reduced rate of?
  • Growth on EMB (positive result for indicator)

Growth

The authors compared the ability of different strains to ferment different sugars

  • ferment xylose slowly compared to control

Plating Assay

plated on eosin-methylene blue agar with 1% xylose

Escherichia coli

NCBI:562

W-45

  • Abolished carbon source utilization (lactose)
  • Abolished lactose catabolic process
  • Growth on EMB (negative result on indicator)

Growth

  • cannot utilize lactose
  • deficient in lactose metabolism

Plating Assay

plated on eosin-methylene blue agar

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Notes