Southern Blot Analysis

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Purpose

This protocol is used to immobilize DNA fragments so the DNA can be used for Hybridization Analysis.

Materials Needed

-DNA sample

-0.25 MHCl

-Denaturation Solution (1.5 M NaCl/0.5 M NaOH)

-Neutralization Solution (1.5 M NaCl/0.5 M Tris-Cl)

-20X and 2X SSC

-Sponge (larger than gel to be blotted)

-Whatman 3 MM filter paper sheets

-Nylon membrane

-UV-transparent plastic wrap

-UV transilluminator

Papers where this or a similar method has been used

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References

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