PMID:810485
| Citation |
Brehm, SP, Le Hegarat, F and Hoch, JA (1975) Deoxyribonucleic acid-binding proteins in vegetative Bacillus subtilis: alterations caused by stage O sporulation mutations. J. Bacteriol. 124:977-84 |
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| Abstract |
Deoxyribonucleic acid (DNA)-binding proteins have been compared between wild-type Bacillus subtilis and five sporulation mutants blocked at different stage O loci. Extracts from exponentially growing cells have been fractionated for proteins binding to single-stranded calf thymus DNA-cellulose and double-stranded B. subtilis DNA-cellulose. In nutrient broth, stage O mutations cause an accumulation of proteins with affinity for double-stranded DNA. Suppression of the mutation with extragenic suppressors relieves the accumulation. In minimal glucose medium, the stage O mutations also cause accumulation of proteins with affinity for double-stranded DNA, but the species accumulated are different from those of nutrient broth-grown cells. In neither case did stage O mutations affect proteins with affinity for single-stranded DNA. The results suggest that the products of stage O loci are functional and operative during vegetative growth. |
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| Keywords |
Bacillus subtilis/growth & development; Bacillus subtilis/metabolism; Bacterial Proteins/metabolism; Culture Media; DNA/metabolism; DNA, Bacterial/metabolism; DNA, Single-Stranded/metabolism; Glucose/metabolism; Molecular Weight; Mutation; Protein Binding; Spores, Bacterial/growth & development; Suppression, Genetic |
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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