id: ECO:0001819
name: primer extension assay evidence
def: "A type of transcript expression level evidence used to determine expression levels of mRNA where a radiolabeled synthetic oligonucleotide primer is annealed to mRNA at the transcription start site of a gene and the mRNA is extended with reverse transcriptase producing cDNA which is electrophoresed on a polyacrylamide gel and autoradiographed, and the band size is proportional to the initial RNA." [ECO:SW, PMID:23378648]
is_a: ECO:0000048 ! transcript expression level evidence
comment: The most commonly used radiolabel is 32P.
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