PMID:330498
| Citation |
Komeda, Y, Silverman, M and Simon, M (1977) Genetic analysis of Escherichia coli K-12 region I flagellar mutants. J. Bacteriol. 131:801-8 |
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| Abstract |
Flagellar mutants in Escherichia coli region I were obtained by selection for resistance to the flagellotropic phage chi. F' elements carrying this region of the E. coli genome were then constructed. Stable merodiploid strains with a flagellar defect on the exogenate and another on the endogenote were prepared. These merodiploids yielded information on the complementation behavior of mutations in this region. Region I was shown to include at least six cistrons, flaV, flaK, flaL, flaM, flaS, and flaT. Mu-induced and deletion fla mutants were also isolated. By using these mutant strains, the transcriptional order was shown to be flaV-flaK-flaL-flaM-flaS-flaT. The definition of region I fla genes and their transcriptional relationships were confirmed by genetic tests with hybrid A phage carrying fla genes in this region. |
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| Keywords |
Chromosome Mapping; Coliphages/growth & development; Escherichia coli/ultrastructure; Flagella/ultrastructure; Genes; Genetic Complementation Test; Lysogeny; Mutation; Operon; Transduction, Genetic |
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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