PMID:27573014
| Citation |
'Jorgenson, MA and Young, KD (2016) Interrupting biosynthesis of O-antigen or the lipopolysaccharide core produces morphological defects in Escherichia coli by sequestering undecaprenyl phosphate. J. Bacteriol. ' |
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| Abstract |
Undecaprenyl phosphate (Und-P) is a member of the family of essential polyprenyl phosphate lipid carriers and, in the Gram-negative bacterium Escherichia coli, is required for synthesizing the peptidoglycan (PG) cell wall, enterobacterial common antigen (ECA), O-antigen and colanic acid. Previously, we found that interrupting ECA biosynthesis indirectly alters PG synthesis by sequestering Und-P via dead-end intermediates, causing morphological defects. To determine if competition for Und-P was a more general phenomenon, we determined if O-antigen intermediates caused similar effects. Indeed, disrupting the synthesis of O-antigen or LPS core oligosaccharide induced cell shape deformities, which were suppressed by preventing the initiation of O-antigen biosynthesis or by manipulating Und-P metabolism. We conclude that accumulating O-antigen intermediates alters PG synthesis by sequestering Und-P. Importantly, many previous experiments addressed the physiological functions of various oligosaccharides and glyconjugates, but these studies employed mutants that accumulate deleterious intermediates. Thus, conclusions based on these experiments must be re-evaluated to account for possible indirect effects of Und-P sequestration. |
| Links |
PubMed Online version:10.1128/JB.00550-16 |
| Keywords |
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