Category:ECO:0005800 ! dilution assay evidence

id: ECO:0005800 name: dilution assay evidence def: "A direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response." [1] synonym: "critical dilution assay" EXACT [] synonym: "spot assay" EXACT [] is_a: ECO:0000002 ! direct assay evidence comment: For chemicals, bacteriocins, or phage, the indicator culture is typically present in a soft-agar layer on top of nutrient agar and a small quantity of each dilution is spotted onto the soft agar overlay.	<obo_figure/>
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