PMID:128555

Citation	Mizuno, T, Yamada, H, Yamagata, H and Mizushima, S (1976) Coordinated alterations in ribosomes and cytoplasmic membrane in sucrose-dependent, spectinomycin-resistant mutants of Escherichia coli.J. Bacteriol. 125:524-30
Abstract	Alterations in cytoplasmic membrane and ribosomes from sucrose-dependent spectinomycin-resistant (Sucd-Spcr) mutants of Escherichia coli, mutants that are resistant to spectinomycin in the presence of 20% sucrose but sensitive in the absence of sucrose, were studied. The protein composition of cytoplasmic membrane was analyzed by gel electrophoresis on polyacrylamide gel containing 8 M urea and 0.5% sodium dodecyl sulfate, which assured the reproducible separation of 28 protein bands. A major protein band, I-19, was missing in all cytoplasmic membrane preparations from 10 Sucd-Spcr mutants. Besides protein I-19, proteins I-13 and I-24 were missing in some mutants. On the other hand, the protein composition of cytoplasmic membrane from a sucrose-independent spectinomycin-resistant mutant was indistinguishable from that from the wild-type strain. The polypeptide synthetic activity of ribosomes from Sucd-Spcr mutants was resistant to spectinomycin. Studies on a revertant obtained from one of these mutants without any selection for sensitivity to spectinomycin revealed that a single mutation was responsible for both the ribosomal alteration, i.e., spectinomycin resistance, and the lack of protein I-19 in the cytoplasmic membrane. Studies on a transductant obtained with a Sucd-SPcr mutant as the donor also confirmed the single-mutation concept. It was concluded that in Sucd-SPcr mutants an alteration in the ribosomes caused the deletion of protein I-19 from cytoplasmic membrane.
Links	PubMed
Keywords	Bacterial Proteins; Cell Membrane; Cell-Free System; Drug Resistance, Microbial; Escherichia coli; Mutation; Peptide Biosynthesis; Ribosomes; Spectinomycin; Sucrose
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: YM50 NCBI Taxon ID: 83333	Genotype of Reference Strain: Suc+-Spc+ Genotype of Experimental Strain : Sucd-Spcr in YM50	Reference Condition:					The ribosomal alteration that causes spectinomycin also caused the protein I-19 to be deleted. This was also detected in strains YM22, YM26, YM37, YM54, YM69, YM84, YM85, YM93 & YM101. See table 2.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: YM101 NCBI Taxon ID: 83333	Genotype of Reference Strain: Suc+-Spc+ Genotype of Experimental Strain : Sucd-Spcr in YM101	Reference Condition:					The ribosomal alteration that causes spectinomycin also caused the protein I-13 to be deleted. See table 2 and Figure 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: YM22 NCBI Taxon ID: 83333	Genotype of Reference Strain: Suc+-Spc+ Genotype of Experimental Strain : Sucd-Spcr in YM22	Reference Condition:					The ribosomal alteration that causes spectinomycin also caused the protein I-24 to be deleted. I-24 was deleted in stains YM50 & YM93. See table 2.
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