id: ECO:0000118
name: subtractive hybridization evidence
def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187]
synonym: "IEP: subtractive hybridization" RELATED []
is_a: ECO:0000009 ! transcript expression evidence
is_a: ECO:0000026 ! nucleic acid hybridization evidence
comment: Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries.
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