PMID:387748
| Citation |
Dabbs, ER (1979) Selection for Escherichia coli mutants with proteins missing from the ribosome.J. Bacteriol. 140:734-7 |
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| Abstract |
Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing. |
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| Keywords |
Bacterial Proteins; Escherichia coli; Genes; Mutation; Ribosomal Proteins |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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It was found that the S20 ribosomal protein was missing indicating that there was a loss of dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. List of mutants include AM13, AM30, AM101, AM106 & AM107 (table 1). See figure 1. |
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a mutation or genetic difference within a strain |
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It was found that the L11 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. Mutants include AM68, AM76 and AM77 (table 1.) See figure 1. |
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a mutation or genetic difference within a strain |
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It was found that the L15 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. There was one mutant, AM16(Table 1). See Figure 1. |
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a mutation or genetic difference within a strain |
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It was found that the L28 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. Mutants include AM3, AM21, AM81 and AM108 (table 1). See Figure 1 |
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a mutation or genetic difference within a strain |
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It was found that the L29 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. A known mutant is AM111(see table 1). See figure 1 |
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a mutation or genetic difference within a strain |
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It was found that the L30 ribosomal protein was missing indicating that there was a loss of translation dependence on Erythromycin. It is unknown whether this caused renewed sensitivity toward Erthyromycin. There were 3 mutants AM10, AM46, AM98 (see table 1.) See Figure 1. |
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Notes
References
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