PMID:6394957
| Citation |
Boeke, JD, LaCroute, F and Fink, GR (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.Mol. Gen. Genet. 197:345-6 |
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| Abstract |
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells. |
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| Keywords |
Carboxy-Lyases; Drug Resistance, Microbial; Fungal Proteins; Microbiological Techniques; Orotic Acid; Orotidine-5'-Phosphate Decarboxylase; Saccharomyces cerevisiae |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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ECO:0000182 |
in vitro culture assay data |
Resistant to Fluoro(5)orotic acid |
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Notes
Waiting on OMP new term. Orotidine-5-phosphate resistance (not an antibiotic).
References
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