PMID:346560

Citation	Pugsley, AP and Schnaitman, CA (1978) Outer membrane proteins of Escherichia coli. VII. Evidence that bacteriophage-directed protein 2 functions as a pore.J. Bacteriol. 133:1181-9
Abstract	Protein 1, a major protein of the outer membrane of Escherichia coli, has been shown to be the pore allowing the passage of small hydrophilic solutes across the outer membrane. In E. coli K-12 protein 1 consists of two subspecies, 1a and 1b, whereas in E. coli B it consists of a single species which has an electrophoretic mobility similar to that of 1a. K-12 strains mutant at the ompB locus lack both proteins 1a and 1b and exhibit multiple transport defects, resistance to toxic metal ions, and tolerance to a number of colicins. Mutation at the tolF locus results in the loss of 1a, in less severe transport defects, and more limited colicin tolerance. Mutation at the par locus causes the loss of protein 1b, but no transport defects or colicin tolerance. Lysogeny of E. coli by phage PA-2 results in the production of a new major protein, protein 2. Lysogeny of K-12 ompB mutants resulted in dramatic reversal of the transport defects and restoration of the sensitivity to colicins E2 and E3 but not to other colicins. This was shown to be due to the production of protein 2, since lysogeny by phage mutants lacking the ability to elicit protein 2 production did not show this effect. Thus, protein 2 can function as an effective pore. ompB mutations in E. coli B also resulted in loss of protein 1 and similar multiple transport defects, but these were only partially reversed by phage lysogeny and the resulting production of protein 2. When the ompB region from E. coli B was moved by transduction into an E. coli K-12 background, only small amounts of proteins 1a and 1b were found in the outer membrane. These results indicate that genes governing the synthesis of outer membrane proteins may not function interchangeably between K-12 and B strains, indicating differences in regulation or biosynthesis of these proteins between these strains.
Links	PubMed
Keywords	Bacterial Proteins; Cell Membrane Permeability; Colicins; Coliphages; Drug Resistance, Microbial; Escherichia coli; Genes; Lysogeny; Membrane Proteins
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 834 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000182	in vitro culture assay data	chlormaphenicol resistant
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 834 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:			ECO:0000182	in vitro culture assay data	copper resistance
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin A
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin E1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin E2
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin E3
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin K
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin L
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin S4
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to colicin X
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to phage hy2
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to phage Me1
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CS197 NCBI Taxon ID: 83333	Genotype of Reference Strain: ompR Genotype of Experimental Strain : ompR	Reference Condition:					Resistant to phage tuIb
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Notes

Needs phage resistance phenotype. Not submitted yet.

References

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