PMID:4189957

Citation	Wistreich, G and Bartholomew, JW (1969) The binding of crystal violet by isolated bacterial cell-wall material. J. Gen. Microbiol. 59:223-7
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Bacillus subtilis; Bacteria; Binding Sites; Cell Wall; Clostridium; Enterobacter; Escherichia coli; Gentian Violet; Micrococcus; Pseudomonas; Sarcina; Serratia marcescens; Staining and Labeling
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Main Points of the Paper

Please summarize the main points of the paper.

Isolated bacterial wall material bound crystal violet as well as or better than intact bacteria
There was no correlation between its ability to bind dye and the Gram characteristic of the intact bacteria
The ability of bacterial wall material to bind crystal violet varied considerably between bacterial species

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Strains:

Gram-positive organisms
- Bacillus subtilis
- Clostridium sporogenes
- Micrococcus roseus
- Sarcina lutea

Gram-negative organisms
- Aerobacter aerogenes
- Escherichia coli
- Pseudomonas fluorescens
- Serratia marcescens

Growth media descriptions
- Trypticase Soy broth (all strains but C. sporogenes)
- Thioglycollate broth (C. sporogenes)
- Butyric acid (for B. subtilis to prevent sporulation)

Cell Wall Preparation
- Intact bacteria were disrupted using the synthetic zeolite procedure
- Cell wall fragments were isolated
- Dry weights determined by drying 2ml volumes of the wall suspensions at 105˚C to constant weight

Determination of dye uptake
- used crystal violet
- dye concentration kept constant in a test series, decreasing the amount of wall material

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
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