PMID:4381707
| Citation |
Bertino, JB and Stacey, KA (1966) A suggested mechanism for the selective procedure for isolating thymine-requiring mutants of Escherichia coli. Biochem. J. 101:32C-33C |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Anti-Bacterial Agents/pharmacology; Escherichia coli/enzymology; Ligases/metabolism; Molecular Biology; Mutation; Nucleotides; Streptococcus pneumoniae; Tetrahydrofolate Dehydrogenase/metabolism; Thymine |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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Notes
- It was found that an altered dihydrofolate can not be adduced wild type thy+ and thy- extracts were examined. It was found that that the Km values for both their dihydrofolate-reductase activity was nearly the same (figure 1) as where the effects of KCl, CaCl2 and other organic mercurials (Table 1) as was the overall activity levels of dihydrofolaate-reductase. Serine hydroxymethylase and N5N10-methylene dehydroxgenase activities were also similar between the wild type and the mutant.
- The correlation between the loss of thymidylate synthetase and resistance to inhibitors of dihydrofolate reductase may be due to the one striking difference between thymidylate synthetase.
- thy- mutants 15T- and B3 were found to be resistant to trimethoprim though 15T- was found to more resistant, the main difference between them being that 15T- has no thymidylate synthetase activity while B3 had at least 20 percent of the normal activity.
References
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