PMID:350837
| Citation |
Duncan, BK , Rockstroh, PA and Warner, HR (1978) Escherichia coli K-12 mutants deficient in uracil-DNA glycosylase. J. Bacteriol. 134:1039-45 |
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| Abstract |
A new assay specific for uracil-DNA glycosylase is described, Escherichia coli mutants partially and totally deficient in uracil-DNA glycosylase activity have been isolated by using this assay in mass-screening procedures. These have been designated ung mutants. The ung gene maps between tyrA and nadB on the E. coli chromosome. T4 phage containing uracil in their DNA grow on the most glycosylase-deficient hosts but are unable to grow on wild-type bacteria. This provides a simple spot test for the ung genotype. The ung mutants show slightly higher rates of spontaneous mutation to antibiotic resistance. Taken together, these results suggest a central role for uracil-DNA glycosylase in the initiation of an excision repair pathway for the exclusion of uracil from DNA. |
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| Keywords |
Chromosome Mapping; Coliphages/analysis; Coliphages/growth & development; DNA Repair; DNA, Viral/analysis; Deoxyuracil Nucleotides; Escherichia coli/genetics; Genes; Mutation; N-Glycosyl Hydrolases/genetics; Uracil/analysis |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000298 |
enzyme activity phenotype |
0000181 |
in vitro assay evidence |
ung mutation caused reduced Uracil-DNA glycosylase activity at 42 o C. Also seen in strain BD13. See table 2. |
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Notes
References
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