PMID:6389496
| Citation |
Matsuzawa, H , Ushiyama, S , Koyama, Y and Ohta, T (1984) Escherichia coli K-12 tolZ mutants tolerant to colicins E2, E3, D, Ia, and Ib: defect in generation of the electrochemical proton gradient. J. Bacteriol. 160:733-9 |
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| Abstract |
Spontaneous Escherichia coli K-12 mutants tolerant to colicin E3 were isolated, and on the basis of their tolerance patterns to 19 kinds of colicins, a new phenotypic class of tolZ mutants was found. The tolZ gene was located between min 77 and 78 on the E. coli K-12 genetic map. The tolZ mutants were tolerant to colicins E2, E3, D, Ia, and Ib, and showed an increased sensitivity to ampicillin, neomycin, and EDTA, but not to deoxycholate; they were able to grow on glucose minimal medium, but not on nonfermentable carbon sources (succinate, acetate, pyruvate, lactate, malate, etc.). The pleiotropic phenotype of the tolZ mutant was due to a single mutation. Both respiration and membrane ATPase activity of the tolZ mutant were normal. The tolZ mutant had a defect in the uptake of proline, glutamine, thiomethyl-beta-D-galactoside, and triphenylmethylphosphonium ion; these uptake systems are driven by an electrochemical proton gradient (delta-mu H+) or a membrane potential (delta psi). In contrast, the uptake of methionine and alpha-methyl-D-glucoside, which is not dependent on delta-mu H+ and delta psi, was normal in the tolZ mutant. Glucose 6-phosphate uptake at pH 5.5, which is driven by a transmembrane pH gradient, in the tolZ mutant was similar to the parent level. These results indicate that the tolZ mutant has a defect in the generation of delta-mu H+ and delta psi. |
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| Keywords |
Ampicillin/pharmacology; Anaerobiosis; Biological Transport, Active/drug effects; Colicins/pharmacology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Genetic Markers; Genotype; Hydrogen-Ion Concentration; Membrane Potentials/drug effects; Mutation; Neomycin/pharmacology; Phenotype; Species Specificity; Structure-Activity Relationship |
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Main Points of the Paper
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Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
Mutation to the tolZ gene conferred resistance to the colicin E2. This resistance can also be seen in strains UM31, UM34 & UM37. See Table 3. |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
Mutation to the tolZ gene conferred resistance to the colicin E3. This resistance can also be seen in strains UM31, UM34 & UM37. See Table 3. |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
Mutation to the tolZ gene conferred resistance to the colicin D. This resistance can also be seen in strains UM31, UM34 & UM37. See Table 3. |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
Mutation to the tolZ gene conferred resistance to the colicin Ia. This resistance can also be seen in strains UM31, UM34 & UM37. See Table 3. |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
Mutation to the tolZ gene conferred resistance to the colicin Ib. This resistance can also be seen in strains UM31, UM34 & UM37. See Table 3. |
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a mutation or genetic difference within a strain |
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It was found the tolZ conferred an increase sensitivity toward the aminoglycoside Ampicillin. This was also seen in strains UM34 & UM37. See Table 5 |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
Mutation to the tolZ gene conferred resistance to the colicin S1. This resistance can also be seen in strains UM31, UM34 & UM37. See Table 3. |
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a mutation or genetic difference within a strain |
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It was found the tolZ conferred an increase sensitivity toward the Neomycin. This was also seen in strains UM34 & UM37. See Table 5 |
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a mutation or genetic difference within a strain |
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It was found the tolZ conferred an increase sensitivity toward EDTA. This was also seen in strains UM34 & UM37. See Table 5 |
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a mutation or genetic difference within a strain |
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The tolZ mutation causes reduced growth under aerobic conditions compared to the wild type, specifically 45 percent of it. See figure 1 for experimental data. |
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a mutation or genetic difference within a strain |
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The tolZ mutation caused decrease uptake of the various amino acids including Proline, Glutamine & Methionine. See Figure 2. |
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a mutation or genetic difference within a strain |
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The tolZ mutation causes reduced growth under anaerobic conditions compared to the wild type, specifically 60 percent of it. See figure 1 for experimental data. |
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a mutation or genetic difference within a strain |
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The tolZ mutation caused decreased uptake of Thiomethyl-beta-D-galactoside & alpha-methyl-D-glucoside. Figure 2. |
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a mutation or genetic difference within a strain |
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The tolZ mutation caused a decrease in the uptake of triphenylmethylphosphonium. See Figure 2. |
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Notes
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