PMID:217866

Citation	Aono, R, Yamasaki, M and Tamura, G (1979) High and selective resistance to mecillinam in adenylate cyclase-deficient or cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli.J. Bacteriol. 137:839-45
Abstract	Adenylate cyclase-deficient (cya) mutants of Escherichia coli K-12 were selectively and highly resistant to mecillinam (FL1060) among several beta-lactam antibiotics in the absence of cyclic adenosine 3',5'-monophosphate (cAMP). They became sensitive to the drug in the presence of cAMP. Also, cAMP receptor protein-negative (crp) mutants, with the exception of strain 5333, were highly resistant to mecillinam in the presence and in the absence of cAMP. Mecillinam exerted two distinct and sequential effects in both cya+ strains and cya strains supplemented with cAMP: (i) rounding of cells and (ii) cessation of cell division. The first effect was accompanied by a decrease in growth rate, whereas the second effect was accompanied by enlargement and lysis of the rounded cells. The second effect of mecillinam was dependent on inoculum size and cAMP. When the cell density was above about 10(6) cells per ml, the rounded cells stopped dividing but did not lyse. In the absence of cAMP, cya strains neither stopped dividing nor lysed; they were resistant to the second, lethal effect of mecillinam.
Links	PubMed
Keywords	Adenylate Cyclase; Amdinocillin; Cyclic AMP; Escherichia coli; Mutation; Penicillanic Acid; Penicillin Resistance; Receptors, Cyclic AMP
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: A010 NCBI Taxon ID: 83333	Genotype of Reference Strain: crp Genotype of Experimental Strain : crp -	Reference Condition:	0000276	increased resistance to antimicrobial compound			Resistance to Mecillinam
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Notes

ECOID: Smi Solid ID is awaiting approval.

OMP term: is an extended-spectrum penicillin antibiotic, so term is awaiting approval.

References

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