PMID:4570603

Citation	Sparling, PF, Ikeya, Y and Elliot, D (1973) Two genetic loci for resistance to kasugamycin in Escherichia coli.J. Bacteriol. 113:704-10
Abstract	There are two loci for resistance to the antibiotic kasugamycin (Ksg) in Escherichia coli. Mutations at ksgA resulted in 30S ribosomal subunit resistance to Ksg. The map location of ksgA was near minute 0.5: ksgA was 95% cotransducible with pdxA, and the apparent gene order was thr... ksgA... pdxA. Studies in stable ksgA/ksgA(+) merodiploids showed that sensitivity was dominant over resistance. Mutations at a second gene (ksgB), located between minutes 25 and 39, resulted in phenotypic Ksg(R) indistinguishable from ksgA mutations, but ribosomes from ksgB strains were sensitive to the drug in vitro. Spontaneous and induced mutations to Ksg(R) were usually of the ksgA (ribosomal) type.
Links	PubMed
Keywords	Alleles; Anti-Bacterial Agents; Carbon Isotopes; Cell-Free System; Chromosome Mapping; Chromosomes, Bacterial; Conjugation, Genetic; Drug Resistance, Microbial; Escherichia coli; Genes; Glycosides; Mutagens; Mutation; Nitrosoguanidines; Recombination, Genetic; Ribosomes; Transduction, Genetic; Valine
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	Notes
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC12 NCBI Taxon ID: 83333	Genotype of Reference Strain: ksgA ksgB Genotype of Experimental Strain : ksgA23 ksgB1	Reference Condition:	OMP:0000276	increased resistance to antimicrobial compound	Double mutant is Resistant to Kasugamycin at levels greater than 2,000 µg/ml. See table 5.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC411 NCBI Taxon ID: 83333	Genotype of Reference Strain: ksgA Genotype of Experimental Strain : ksgA19	Reference Condition:	OMP:0000276	increased resistance to antimicrobial compound	Resistant to Aminoglycoside Kasugamycin at concentrations at 1,000 µg/ml. Mutant strain: FS232, on table 5.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: JC411 NCBI Taxon ID: 83333	Genotype of Reference Strain: ksgA Genotype of Experimental Strain : ksgA19	Reference Condition:	OMP:0000276	increased resistance to antimicrobial compound	Resistant to Aminoglycoside Kasugamycin at concentrations at 2,000 µg/ml. Mutant strain: FS131, on table 5.
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