PMID:2540960

Citation	Bouloc, P, Jaffé, A and D'Ari, R (1989) The Escherichia coli lov gene product connects peptidoglycan synthesis, ribosomes and growth rate.EMBO J. 8:317-23
Abstract	Mecillinam, a beta-lactam antibiotic which binds specifically to penicillin-binding protein 2 (PBP2), blocks lateral cell-wall elongation, induces spherical morphology and ultimately kills bacteria. We describe here a new mecillinam-resistant mutant of Escherichia coli, the lov mutant. It possesses active PBP2, as evidenced by its rod shape in the absence of mecillinam (but not in its presence), its ability to filament when septation is inhibited, and its penicillin-binding ability. The lov mutant grows slowly but seems to regulate its macromolecular parameters properly: cell volume, RNA content (ribosome concentration), and DNA content are appropriate for the growth rate, and the growth yield is identical to that of wild type. The lov mutation is located at 41 min on the E.coli genetic map and is recessive. Certain rpsL (StrR) mutations suppress the lov mutant's mecillinam resistance. The allele specificity of the suppression suggests that the lov gene product may interact directly with the ribosomes. The lov gene product thus seems to define a link between PBP2 (the mecillinam target) and the ribosomes; we propose that this link is involved in transmitting information on the growth rate (ribosome concentration) to the peptidoglycan synthesizing apparatus.
Links	PubMed
Keywords	Acyltransferases; Amdinocillin; Bacterial Proteins; Carrier Proteins; Cyclic AMP; Depression, Chemical; Drug Resistance, Microbial; Escherichia coli; Genes, Bacterial; Hexosyltransferases; Multienzyme Complexes; Muramoylpentapeptide Carboxypeptidase; Penicillin-Binding Proteins; Peptidoglycan; Peptidyl Transferases; Receptors, Cyclic AMP; Ribosomal Proteins; Ribosomes
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: GC2700 NCBI Taxon ID: 83333	Genotype of Reference Strain: lov Genotype of Experimental Strain : lov-1	Reference Condition:	0000276	increased resistance to antimicrobial compound			Resistance to the penicillin Mecillinam.
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Notes

OMPID: Mecillinam Resistance (a penicillin) term has been submitted and needs to be added.

ECOID: Author trace statement.

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