id: ECO:0000068
name: yeast 2-hybrid evidence
def: "Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed." [TAIR:TED]
synonym: "IPI: yeast two-hybrid assay" RELATED []
is_a: ECO:0000025 ! hybrid interaction evidence
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