Category:ECO:0000076 ! far-Western blotting evidence

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id: ECO:0000076

name: far-Western blotting evidence
def: "Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column." [TAIR:TED]
synonym: "IPI: far-Western analysis" RELATED []
xref: PSI-MI:MI\:0047
is_a: ECO:0000021 ! physical interaction evidence

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