id: ECO:0000226
name: chromatin immunoprecipitation evidence
def: "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC]
synonym: "ChIP evidence" EXACT []
xref: PSI-MI:MI\:0402 "chromatin immunoprecipitation assay"
is_a: ECO:0000085 ! immunoprecipitation evidence
comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).
|
<obo_figure/>
|
| <OBOterm/> |
|
The contents of this box are automatically generated. You can help by adding information to the "Notes"
Usage Notes
References
See Help:References for how to manage references in omp dev.
Child Terms
This category has the following 5 subcategories, out of 5 total.
