id: ECO:0001809
name: DNA affinity chromatography evidence
def: "A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins." [ECO:SW, PMID:11694305, PMID:11725488]
synonym: "DNA affinity purification" RELATED []
is_a: ECO:0000325 ! chromatography evidence
relationship: OBI:0000312: OBI:0001630 ! is_specified_output_of analytical chromatography
comment: Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture.
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