PMID:11689018

Citation	Oh, TJ, Jung, IL and Kim, IG (2001) The Escherichia coli SOS gene sbmC is regulated by H-NS and RpoS during the SOS induction and stationary growth phase.Biochem. Biophys. Res. Commun. 288:1052-8
Abstract	sbmC, an Escherichia coli gene, belongs to the SOS regulon, whose product is involved in cell susceptibility to microcin B17 and its expression is induced at the onset of the stationary growth phase. In the present work, we have investigated the regulation of sbmC expression during SOS induction and the stationary growth phase using a single-copy sbmC'-'lacZ fusion. The SOS induction of sbmC is profoundly diminished in the hns mutant and less diminished in the rpoS mutant. The strain with hns, rpoS double mutation, showed a similar level of sbmC induction to that of a strain with hns single mutation. Mutation in rpoS or hns causes the repression of the sbmC gene during the stationary growth phase. The sbmC expression in the rpoS mutant strain was approximately twofold lower than that in the hns mutant and the rpoS hns double mutant showed a similar level of sbmC expression to mutants deficient in rpoS alone. Interestingly, the sbmC'-'lacZ expression in the exponential growth phase was not derepressed in the hns mutant background. Transformation of hns and rpoS mutants with plasmids carrying histone-like nucleoid protein (H-NS) and RpoS effectively restored the sbmC expression to the wild-type level, respectively. The gel mobility shift assay showed that purified H-NS protein directly bound with a high affinity to a DNA fragment carrying the sbmC promoter region. These findings demonstrate that H-NS regulates the sbmC expression via H-NS's direct binding to the promoter region. In conclusion, our data suggest that H-NS and RpoS regulate a stationary phase-inducible sbmC gene in E. coli.
Links	PubMed Online version:10.1006/bbrc.2001.5872
Keywords	Bacterial Proteins; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetic Complementation Test; Genotype; Mutation; Promoter Regions, Genetic; Protein Binding; Recombinant Fusion Proteins; Repressor Proteins; SOS Response (Genetics); Sigma Factor
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: hns Genotype of Experimental Strain : hns-206::amp	Reference Condition:					Inactivation of hns strongly reduced the SOS induction of sbmC compared to the wild type, fig 1B. same applies to double mutant hns rpoS.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: rpoS Genotype of Experimental Strain : rpoS359::Tn10	Reference Condition:					Inactivation of the rpoS gene reduced the SOS induction of the sbmC gene, figure 1A.
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