PMID:21196

Citation	DuPree, MR and Wilcox, G (1977) Bromothymol blue and carbohydrate-sensitive plating media.J. Clin. Microbiol. 6:343-7
Abstract	A new plating medium using bromothymol blue (BTB) indicator is described and compared with eosin-methylene blue (EMB), MacConkey, and Endo media. These media were tested with L-arabinose by plating fermenting and nonfermenting mutant strains of Escherichia coli. The minimum concentrations of L-arabinose that permitted differentiation of these strains were determined. Different concentrations were required for differentiating confluent patches of cells, isolated colonies, and closely spaced or adjacent colonies. L-Arabinose, L-rhamnose, D-lactose, and D-galactose were tested with modified enteric media and with BTB medium, again to determine minimum usable concentrations. BTB media and reformulated conventional media allowed detection of acidification, aerobically, at one-fifth to one-hundredth the (1%, wt/vol) concentration of carbohydrate used in standard indicator plates.
Links	PubMed
Keywords	Aerobiosis; Arabinose; Bacteria; Bromthymol Blue; Carbohydrate Metabolism; Culture Media; Escherichia coli; Hydrogen-Ion Concentration; Indicators and Reagents; Mutation; Peptones; Stereoisomerism; Thymol
edit table

Main Points of the Paper

Please summarize the main points of the paper.

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

See Help:AnnotationTable for details on how to edit this table.
<protect>

Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
edit table

</protect>

See Help:References for how to manage references in omp dev.