PMID:22047137
| Citation |
Lee, JH, Park, JH, Kim, JA, Neupane, GP, Cho, MH, Lee, CS and Lee, J (2011) Low concentrations of honey reduce biofilm formation, quorum sensing, and virulence in Escherichia coli O157:H7. Biofouling 27:1095-104 |
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| Abstract |
Bacterial biofilms are associated with persistent infections due to their high resistance to antimicrobial agents. Hence, controlling pathogenic biofilm formation is important in bacteria-related diseases. Honey, at a low concentration of 0.5% (v/v), significantly reduced biofilm formation in enterohemorrhagic Escherichia coli O157:H7 without inhibiting the growth of planktonic cells. Conversely, this concentration did not inhibit commensal E. coli K-12 biofilm formation. Transcriptome analyses showed that honey significantly repressed curli genes (csgBAC), quorum sensing genes (AI-2 importer and indole biosynthesis), and virulence genes (LEE genes). Glucose and fructose in the honeys were found to be key components in reducing biofilm formation by E. coli O157:H7 through the suppression of curli production and AI-2 import. Furthermore, honey, glucose and fructose decreased the colonization of E. coli O157:H7 cells on human HT-29 epithelial cells. These results suggest that low concentrations of honey, such as in honeyed water, can be a practical means for reducing the colonization and virulence of pathogenic E. coli O157:H7. |
| Links |
PubMed Online version:10.1080/08927014.2011.633704 |
| Keywords |
Bacterial Proteins/metabolism; Biofilms; Escherichia coli K12/physiology; Escherichia coli O157/pathogenicity; Escherichia coli O157/physiology; Fructose; Gene Expression Regulation, Bacterial; Glucose; HT29 Cells; Honey; Humans; Quorum Sensing; Virulence |
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