PMID:3084791
| Citation |
Gillespie, J , Barton, LL and Rypka, EW (1986) Phenotypic changes in mycobacteria grown in oxygen-limited conditions. J. Med. Microbiol. 21:251-5 |
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| Abstract |
Laboratory strains of Mycobacterium phlei, M. smegmatis, M. fortuitum, M. gordonae, M. kansasi, M. bovis, M. tuberculosis and M. intracellulare were adapted to grow in an anaerobic environment. Concomitant with the transition to anaerobic growth was loss of acid-fastness, loss or modification of colonial pigmentation, and loss of ability to grow on a malachite green-containing medium. The mycobacteria grown anaerobically produced acid from a greater range of carbohydrates than aerobically grown cultures, lost iron-uptake activity, and showed a reduction of urease, catalase and nitratase activity. Back adaption of mycobacteria from an anaerobic to an aerobic environment resulted in the acquisition of acid-fastness, pigmentation, and other characteristics used in the taxonomy of mycobacteria. These results suggest that mycobacterial cultures, if grown in an anaerobic environment, may be erroneously identified in clinical laboratories. |
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| Keywords |
Adaptation, Physiological; Aerobiosis; Anaerobiosis; Carbohydrate Metabolism; Carotenoids/biosynthesis; Culture Media; Mycobacterium/cytology; Mycobacterium/growth & development; Mycobacterium/physiology; Mycobacterium bovis/cytology; Mycobacterium bovis/growth & development; Mycobacterium bovis/physiology; Mycobacterium phlei/cytology; Mycobacterium phlei/growth & development; Mycobacterium phlei/physiology; Mycobacterium tuberculosis/cytology; Mycobacterium tuberculosis/growth & development; Mycobacterium tuberculosis/physiology; Nontuberculous Mycobacteria/cytology; Nontuberculous Mycobacteria/growth & development; Nontuberculous Mycobacteria/physiology; Phenotype; Rosaniline Dyes/pharmacology; Sodium Chloride/pharmacology; Temperature |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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Notes
References
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