PMID:4588694
| Citation |
Kennedy, EP , Rumley, MK and Armstrong, JB (1974) Dierect measurement of the binding of labeled sugars to the lactose permease M protein. J. Biol. Chem. 249:33-7 |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Bacterial Proteins/metabolism; Cell Membrane/drug effects; Cell Membrane/enzymology; Cell-Free System; Chromatography, Ion Exchange; Escherichia coli/cytology; Escherichia coli/enzymology; Galactose; Hydrogen-Ion Concentration; Isotope Labeling; Kinetics; Lactose; Membrane Transport Modulators; Membrane Transport Proteins/antagonists & inhibitors; Membrane Transport Proteins/metabolism; Methods; Mutation; Phosphorus Radioisotopes; Receptors, Drug; Structure-Activity Relationship; Surface-Active Agents/pharmacology; Thioglycosides/metabolism; Time Factors; Tritium |
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Main Points of the Paper
Please summarize the main points of the paper.
- Describes a method for measuring the binding of labeled sugars to M (LacY) directly
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- Strain: A-324 (pro-lac i-z+y+F-)
- Strain: A-3244 (pro-lac i-z+y+/F' pro+lac i+z+y+)
- Strain: A-3245 (pro-lac i-z+y+F' pro+lac i-z+y+)
- Strain: ML308 (lac i-z+y+)
- Strain: ML308-225 (lac i-z-y+)
- Thiodi[3H]galactoside (TDG) binding assays
- Cell-free fractions/ membrane particles
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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Notes
References
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