PMID:4935495

Citation	von Graevenitz, A (1971) Practical substitution for the indol, methyl red, Voges-Proskauer, citrate system. Appl Microbiol 21:1107-9
Abstract	A substitution for the indole, methyl red, Voges-Proskauer, citrate system is proposed. The scheme employs indole and ampicillin susceptibility tests routinely and uses selectively three other media for six reactions.
Links	PubMed PMC377358
Keywords	Ampicillin/pharmacology; Azo Compounds/metabolism; Bacteriological Techniques; Butanones/biosynthesis; Citrates/metabolism; Enterobacteriaceae/classification; Enterobacteriaceae/drug effects; Enterobacteriaceae/metabolism; Indoles/biosynthesis; Methods; Microbial Sensitivity Tests; Penicillin Resistance
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Main Points of the Paper

Please summarize the main points of the paper.

• A new system for identification is discussed
• Substitutions for current clinical tests must be made because they:
  • are expensive
  • are time-consuming
  • may lead to misidentification

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

• IMViC tests
  • indole
  • methyl red
  • Vogues-Proskauer
  • citrate

• Isolation and Differentiation media
  • MacKonkey
  • deoxycholate
  • endo agar

• Media and tests selectively used for the identification of pink colonies
  • Trypticase for indole-check
  • 10-µg ampicillin disc for Kirby-Bauer sensitivity testing on Mueller-Hinton Agar
  • motility-ornithine medium
  • lysine-iron agar
  • Koser citrate medium
  • Dimethyl-p-phenylenediamine-hydrochloride for the Kovacs oxidase test

• Nonmucoid strains
• Mucoid strains

Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
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References

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