PMID:794878

Citation	Gellert, M, O'Dea, MH, Itoh, T and Tomizawa, J (1976) Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.Proc. Natl. Acad. Sci. U.S.A. 73:4474-8
Abstract	Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a cell-free system is partially relieved by adding resistant DNA gyrase. Both in the case of coliclls. DNA molecules which are converted to the covalently circular form in thepresence of coumermycin remain relaxed, instead of achieving their normal supercoiled conformation. We conclude that DNA gyrase controls the supercoiling of DNA in E. coli.
Links	PubMed
Keywords	Anti-Bacterial Agents; Colicins; Coumarins; DNA Replication; DNA, Bacterial; DNA, Circular; DNA, Viral; Escherichia coli; Mutation; Novobiocin; Nucleic Acid Conformation; Plasmids; Pyrroles
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	Notes
a mutation or genetic difference within a strain	Taxon: Escherichia coli	Genotype of Reference Strain:	Reference Condition:
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Col E1 NCBI Taxon ID: 83333	Genotype of Reference Strain: gyrB⁺ Genotype of Experimental Strain : gyrB mutation	Reference Condition:	As seen in figure 1 (lanes i-n) cou^r was used to confer resistance toward Novobiocin.
a mutation or genetic difference within a strain	Taxon: E-coli Strain: K-12 Substrain: Coli E1 NCBI Taxon ID: 83333	Genotype of Reference Strain: gyrB⁺ Genotype of Experimental Strain : gyrB mutation	Reference Condition: with novobiocin	Refer to fig 1 lanes (o-p), gel electrophoresis. Two enzymes, sensitive (N99 recB) and resistant(NI748), were mixed together and found 50% inhibition of novobiocin.
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