PMID:9405611
| Citation |
Rensing, C , Mitra, B and Rosen, BP (1997) The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase. Proc. Natl. Acad. Sci. U.S.A. 94:14326-31 |
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| Abstract |
The first Zn(II)-translocating P-type ATPase has been identified as the product of o732, a potential gene identified in the sequencing of the Escherichia coli genome. This gene, termed zntA, was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain exhibited hypersensitivity to zinc and cadmium salts but not salts of other metals, suggesting a role in zinc homeostasis in E. coli. Everted membrane vesicles from a wild-type strain accumulated 65Zn(II) and 109Cd(II) by using ATP as an energy source. Transport was sensitive to vanadate, an inhibitor of P-type ATPases. Membrane vesicles from the zntA::kan strain did not accumulate those metal ions. Both the sensitive phenotype and transport defect of the mutant were complemented by expression of zntA on a plasmid. |
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| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Molecular Sequence Data; Sequence Alignment; Zinc/metabolism |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
The insertion of kanamycin cassette caused a decrease in resistance toward Cadmium and Zinc Ions. See figure 2 for experimental data. |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
The zntA mutation conferred a decrease in Zn(II) uptake due to everted membrane vesicles. See Figure 4. |
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a mutation or genetic difference within a strain |
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0000178 |
in vivo assay evidence |
The zntA mutation conferred an increase in Vanadate sensitivity. See Figure 5 for experimental results. |
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Notes
References
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