Category:ECO:0000066 ! yeast one-hybrid evidence

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id: ECO:0000066

name: yeast one-hybrid evidence
def: "A type of hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC, PSI-MI:MI\:0432, TAIR:TED]
synonym: "IPI: yeast one-hybrid assay" RELATED []
xref: [ MI:0432 "one hybrid"]
is_a: ECO:0000025 ! hybrid interaction evidence
comment: The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED).

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