id: ECO:0000074
name: split-ubiquitin assay evidence
def: "A type of physical interaction evidence that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." [PMID:15064465]
synonym: "IPI: split-ubiquitin assay" RELATED []
xref: [ MI:0112 "ubiquitin reconstruction"]
is_a: ECO:0000021 ! physical interaction evidence
comment: The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquitin (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough to reconstitute.TAIR:TED
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