id: ECO:0000096
name: electrophoretic mobility shift assay evidence
def: "A type of direct assay evidence based on an electrophoretic mobility shift of macromolecules, compounds, and proteins where solutions of protein and nucleic acid are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect protein-nucleic acid interactions." [ECO:KIM, ECO:SW, PMID:17703195, TAIR:TED]
synonym: "EMSA: electrophoretic mobility shift assay" EXACT []
synonym: "Gel retardation assay" RELATED [PMID:17703195]
synonym: "IDA: electrophoretic mobility shift assay" RELATED []
xref: [ MI:0413 "electrophoretic mobility shift assay"]
is_a: ECO:0000002 ! direct assay evidence
comment: EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid.
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