id: ECO:0000183
name: cell-free assay evidence
def: "A type of direct assay evidence resulting from studies in which cytoplasmic and/or nuclear cellular components from resting, nonstimulated cells, are isolated by ultracentrifugation to provide molecular machinery that can be used in reactions in the absence of many of the other cellular components for the purpose of in vitro protein synthesis, transcription, DNA replication, etc." [PMID:18453125]
synonym: "in vitro assay" BROAD []
is_a: ECO:0000002 ! direct assay evidence
relationship: IAO:0000136: GO:0008152 ! is about metabolic process
relationship: OBI:0000312: OBI:0000978 ! is_specified_output_of in container assay
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