id: ECO:0000223
name: 454 pyrosequencing evidence
def: "A type of high throughput nucleotide sequencing evidence in which the sequence for a ssDNA is determined by addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand." [EFO:0005016]
synonym: "pyrosequencing" BROAD [OBI:0000730]
is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence
comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.\n\nThe target strand is usually prepared by fragmentation, adaptor ligation, and amplification by emulsion PCR.
|
<obo_figure/>
|
| <OBOterm/> |
|
The contents of this box are automatically generated. You can help by adding information to the "Notes"
Usage Notes
References
See Help:References for how to manage references in omp dev.
Child Terms
This category has the following 2 subcategories, out of 2 total.
