id: ECO:0000231
name: quantitative polymerase chain reaction evidence
def: "A type of DNA detection assay evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes or fluorescent probes for detection and quantification of specific sequences in a DNA sample." [ECO:MCC, ECO:SW]
synonym: "Q-PCR evidence" EXACT []
synonym: "qPCR evidence" EXACT []
synonym: "qRT-PCR evidence" RELATED []
synonym: "quantitative PCR evidence" EXACT []
synonym: "quantitative real-time PCR evidence" EXACT []
synonym: "real time polymerase chain reaction evidence" EXACT []
synonym: "real-time PCR evidence" EXACT []
synonym: "real-time quantitative PCR" EXACT []
is_a: ECO:0005519 ! DNA detection assay evidence
relationship: OBI:0000312: OBI:0000893 ! is_specified_output_of real time polymerase chain reaction assay
comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA.\n\nAlthough qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173).\n\nqPCR can be used to quantitatively determine levels of gene expression.
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