id: ECO:0000233
name: 3C evidence
def: "A type of chromosome conformation-based evidence that is derived by 1) quantifying interactions between a single pair of genomic loci and not multiple interactions and 2) performing polymerase chain reaction (PCR) in the final product quantitation step." [ECO:MCC]
synonym: "3C" EXACT []
synonym: "chromosome conformation capture evidence" EXACT []
synonym: "chromosome conformation capture-PCR evidence" NARROW []
is_a: ECO:0000232 ! chromosome conformation-based evidence
relationship: OBI:0000312: OBI:0002439 ! is_specified_output_of chromosome conformation capture assay
comment: 3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation.
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