id: ECO:0001810
name: DNAse footprinting evidence
def: "A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions." [ECO:SW, PMID:212715, PMID:22194258]
synonym: "DNase protection" RELATED []
is_a: ECO:0000136 ! nucleic acid binding evidence
comment: In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites.
|
<obo_figure/>
|
| <OBOterm/> |
|
The contents of this box are automatically generated. You can help by adding information to the "Notes"
Usage Notes
References
See Help:References for how to manage references in omp dev.
Child Terms
This category has the following 2 subcategories, out of 2 total.
