id: ECO:0001817
name: glutathione S-transferase pull-down assay evidence
def: "A type of protein binding evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions." [ECO:SW, PMID:26096507]
synonym: "GST pull-down assay" EXACT [PMID:26096507]
is_a: ECO:0000024 ! protein binding evidence
comment: The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing.
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