id: ECO:0001825
name: isothermal titration calorimetry evidence
def: "A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction." [ECO:SW, PMID:10527727]
synonym: "ITC" EXACT [PMID:10527727]
is_a: ECO:0000023 ! affinity evidence
is_a: IAO:0000030 ! information content entity
relationship: IAO:0000136: OBI:0001588 ! is about binding
relationship: OBI:0000312: OBI:0001634 ! is_specified_output_of calorimetric binding assay
comment: When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b)).
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