Category:ECO:0005520 ! interferometric reflectance imaging sensor evidence

name: interferometric reflectance imaging sensor evidence
def: "A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels." [ECO:SW, PMID:21587155, PMID:24271115]
synonym: "IRIS" EXACT []
synonym: "Spectral Reflectance Imaging Biosensor" EXACT []
synonym: "SRIB" EXACT []
is_a: ECO:0005516 ! molecule detection assay evidence
comment: Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively.