PMID:10639134

Citation	Rensing, C , Fan, B , Sharma, R , Mitra, B and Rosen, BP (2000) CopA: An Escherichia coli Cu(I)-translocating P-type ATPase. Proc. Natl. Acad. Sci. U.S.A. 97:652-6
Abstract	The copA gene product, a putative copper-translocating P-type ATPase, has been shown to be involved in copper resistance in Escherichia coli. The copA gene was disrupted by insertion of a kanamycin gene through homologous recombination. The mutant strain was more sensitive to copper salts but not to salts of other metals, suggesting a role in copper homeostasis. The copper-sensitive phenotype could be rescued by complementation by a plasmid carrying copA from E. coli or copB from Enterococcus hirae. Expression of copA was induced by salts of copper or silver but not zinc or cobalt. Everted membrane vesicles from cells expressing copA exhibited ATP-coupled accumulation of copper, presumably as Cu(I). The results indicate that CopA is a Cu(I)-translocating efflux pump that is similar to the copper pumps related to Menkes and Wilson diseases and provides a useful prokaryotic model for these human diseases.
Links	PubMed PMC15385
Keywords	Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport; Cadmium/metabolism; Cadmium/pharmacology; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cation Transport Proteins; Cell Membrane/metabolism; Copper/metabolism; Copper/pharmacology; Copper Radioisotopes/diagnostic use; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Mutagenesis; Plasmids/genetics; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Silver/pharmacology; Zinc/metabolism; Zinc/pharmacology
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Main Points of the Paper

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Materials and Methods Used

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• Preparation of Everted Membrane Vesicles (Rensing et al 2000)
• ⁶⁴ Cu Transport Assay (Rensing et al 2000)

Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia Coli Strain: K-12 Substrain: W3110 NCBI Taxon ID: 83333	Genotype of Reference Strain: copA Genotype of Experimental Strain : copA(del) In strain LMG194	Reference Condition:	OMP:0000275	decreased resistance to antimicrobial compound	ECO:0000267	enzyme-linked immunoabsorbent assay evidence	Sensitivity to antimicrobial agent Copper Ion. See growth curve in figure 2.
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Notes

OMP Term: Has been requested for metal resistance.

References

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