PMID:10940054
| Citation |
Zhao, J and Winkler, ME (2000) Reduction of GC --> TA transversion mutation by overexpression of MutS in Escherichia coli K-12.J. Bacteriol. 182:5025-8 |
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| Abstract |
Overexpression of the MutS repair protein significantly decreased the rate of lacZ GC --> TA transversion mutation in stationary-phase and exponentially growing bacteria and in mutY and mutM mutants, which accumulate mismatches between 8-oxoguanine (8-oxoG) and adenine residues in DNA. Conversely, GC --> TA transversion increased in mutL or mutS mutants in stationary phase. In contrast, overexpression of MutS did not appreciably reduce lacZ AT --> CG transversion mutation in a mutT mutant. These results suggest that MutS-dependent repair can correct 8-oxoG:A mismatches in Escherichia coli cells but may not be able to compete with mutation fixation by MutY in mutT mutants. |
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| Keywords |
Adenine; Adenosine Triphosphatases; Bacterial Proteins; Base Pair Mismatch; Cystine; DNA Repair; DNA-Binding Proteins; Escherichia coli; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Guanine; MutS DNA Mismatch-Binding Protein; Mutation; Thymine |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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OMP:0005069 |
decreased mutation frequency |
Overexpression of MutS decreases the rate of GC to TA transversion mutations. |
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a mutation or genetic difference within a strain |
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OMP:0005069 |
decreased mutation frequency |
Overexpression of MutS decreases the rate of GC to TA transversion mutations in a strain lacking MutY. |
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Notes
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