PMID:137231
| Citation |
Mizuno, T, Yamagata, H and Mizushima, S (1977) Interaction of cytoplasmic membrane and ribosomes in Escherichia coli: spectinomycin-induced disappearance of membrane protein I-19.J. Bacteriol. 129:326-32 |
|---|---|
| Abstract |
Incubation of Escherichia coli with spectinomycin caused the disappearance of a major protein from the cytoplasmic membrane. This protein, called "I-19", was not a ribosomal protein. Its disappearance was not a result of the direct action of spectinomycin on the cytoplasmic membrane, but a result of its action on ribosomes. The disappearance was specifically induced by spectinomycin, and other antibiotics such as neomycin, erythromycin, and chloramphenicol had no effect. Although growth was not required for spectinomycin-induced disappearance of protein I-19 from the cytoplasmic membrane, the disappearance was not observed under conditions where protein synthesis was inhibited completely either by the addition of chloramphenicol or by cooling in ice. It is suggested that at least some ribosomes interact with the cytoplasmic membrane and that a modification of the mode of interaction through the action of spectinomycin on ribosomes caused the deletion of membrane protein I-19. |
| Links | |
| Keywords |
Bacterial Proteins; Cell Membrane; Chloramphenicol; Erythromycin; Escherichia coli; Membrane Proteins; Neomycin; Ribosomes; Spectinomycin |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
|
a mutation or genetic difference within a strain |
|
|
|
100 ug of chloramphenicol was added 10 minutes prior to the addition of spectinomycin. This concentration caused a delay in the disappearance of the I-19 protein as seen in Figure 7 A-D. This was thought to be due to the fact that Cell growth was only partially inhibited. |
| ||||
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
