PMID:14009486

Citation	ZABIN, I , KEPES, A and MONOD, J (1962) Thiogalactoside transacetylase. J. Biol. Chem. 237:253-7
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Transferases/chemistry
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Main Points of the Paper

Please summarize the main points of the paper.

• Thiogalactoside transacetylase is identified
• Characterization of the purified protein is also described
  • rate of formation of acetyl-IPTG as a function of time- reaction is linear
  • optimal pH is 7.0 in phosphate buffer, but 8.2 in Tris buffer
  • KM<4x10-5 M for acetyl-CoA
  • Butyryl-CoA was about 10% as effective as acetyl-CoA as donors
  • catalyzes the transfer of acetyl from acetyl-CoA to thiogalactosides
  • divalent cations are not required for the formation of acetyl-IPTG
  • activity is slightly inhibited by 0.001 M p-Chloromercuribenzoate (31% inhibition)
  • essentially all the activity present in the supernatant fraction

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

• Strain: ML308
• Hydroxamate assay
• Sulfhydryl assay

Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
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References

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