PMID:15292217

Citation	Lawhorn, BG , Gerdes, SY and Begley, TP (2004) A genetic screen for the identification of thiamin metabolic genes. J. Biol. Chem. 279:43555-9
Abstract	A genetic screen was developed for the identification of genes related to thiamin biosynthesis and degradation. Genes conferring resistance to bacimethrin or 4-amino-2-trifluoromethyl-5-hydroxymethylpyrimidine were selected from Escherichia coli and Bacillus subtilis genomic libraries. Hits from the selection included the known thiamin biosynthetic genes thiC, thiE, and dxs as well as five genes of previously unknown function (E. coli yjjX, yajO, ymfB, and cof and B. subtilis yveN). The gene products YmfB and Cof catalyze the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate. YmfB also converts thiamin pyrophosphate into thiamin phosphate.
Links	PubMed Online version:10.1074/jbc.M404284200
Keywords	Bacillus subtilis/drug effects; Bacillus subtilis/genetics; Cloning, Molecular; Escherichia coli/drug effects; Escherichia coli/genetics; Genomic Library; Phosphorylation; Pyrimidines/pharmacology; Thiamine/biosynthesis; Thiamine/genetics; Thiamine/metabolism
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
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