PMID:15489430

Citation	Van Dyk, TK, Templeton, LJ, Cantera, KA, Sharpe, PL and Sariaslani, FS (2004) Characterization of the Escherichia coli AaeAB efflux pump: a metabolic relief valve?J. Bacteriol. 186:7196-204
Abstract	Treatment of Escherichia coli with p-hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP, encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ, encoding a protein of the membrane fusion protein family, and yhcR, encoding a small protein without a known or suggested function. The function of the upstream, divergently transcribed gene yhcS, encoding a regulatory protein of the LysR family, in regulating expression of yhcRQP was shown. Furthermore, it was demonstrated that several aromatic carboxylic acid compounds serve as inducers of yhcRQP expression. The efflux function encoded by yhcP was proven by the hypersensitivity to pHBA of a yhcP mutant strain. A yhcS mutant strain was also hypersensitive to pHBA. Expression of yhcQ and yhcP was necessary and sufficient for suppression of the pHBA hypersensitivity of the yhcS mutant. Only a few aromatic carboxylic acids of hundreds of diverse compounds tested were defined as substrates of the YhcQP efflux pump. Thus, we propose renaming yhcS, yhcR, yhcQ, and yhcP, to reflect their role in aromatic carboxylic acid efflux, to aaeR, aaeX, aaeA, and aaeB, respectively. The role of pHBA in normal E. coli metabolism and the highly regulated expression of the AaeAB efflux system suggests that the physiological role may be as a "metabolic relief valve" to alleviate toxic effects of imbalanced metabolism.
Links	PubMed Online version:10.1128/JB.186.21.7196-7204.2004
Keywords	Biological Transport; Escherichia coli; Escherichia coli Proteins; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Microbial Sensitivity Tests; Oligonucleotide Array Sequence Analysis; Operon; Parabens; Substrate Specificity
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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli			yhcS	increased sensitivity to p-hydroxybenzoic acid (pHBA)	Sensitivity to
Escherichia coli			yhcP	increased sensitivity to p-hydroxybenzoic acid (pHBA)	Sensitivity to
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