PMID:16095938
| Citation |
Ito, M, Baba, T, Mori, H and Mori, H (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays.Metab. Eng. 7:318-27 |
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| Abstract |
Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were interrelated by the clustering method of the GeneSpring software. In the resulted dendrogram of genes, a group of genes with known and related function tended to be assembled into a cluster. Our clustering method would be useful for functional assignment of so called y-genes with no apparent function, since the resulted dendrogram could connect y-genes to phenotype and function of well-studied genes. |
| Links |
PubMed Online version:10.1016/j.ymben.2005.06.004 |
| Keywords |
Escherichia coli; Gene Deletion; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genes, Bacterial; Oligonucleotide Array Sequence Analysis; Phenotype; Software |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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unable to respire N-Acetyl-D-glucosamine,a-D-Glucose-1-phosphate and Acetate |
Biolog GN2 plate assay |
Mutant shows defective phenotype under N-Acetyl-D-glucosamine,a-D-Glucose-1-phosphate and Acetate chemicals |
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Notes
References
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