PMID:161156

Citation	Ammer, J, Brennenstuhl, M, Schindler, P, Höltje, JV and Zähner, H (1979) Phosphorylation of streptozotocin during uptake via the phosphoenolpyruvate: sugar phosphotransferase system in Escherichia coli.Antimicrob. Agents Chemother. 16:801-7
Abstract	Mutants of Escherichia coli K-12, Staphylococcus aureus, and Bacillus subtilis defective in the general components (enzyme I, or HPr, or both) of the phosphoenolpyruvate:sugar phosphotransferase system are shown to be resistant to the antibiotic streptozotocin. It is shown here, employing 32P-labeled phosphoenolpyruvate, that wild-type cells of E. coli phosphorylate streptozotocin, whereas with a phosphotransferase system-defective mutant of E. coli the drug is recovered in an unaltered, free form. The internal accumulation of streptozotocin at the steady-state level was about 70 times that of the concentration in the external medium. The antibacterial action of streptozotocin, as well as the uptake of the drug, was inhibited by N-acetyl-D-glucosamine. The uptake of the antibiotic was extremely sensitive to p-chloromercuribenzoate. It is concluded that streptozotocin is taken up by E. coli via the phosphoenolpyruvate:sugar phosphotransferase system and consequently accumulates in the cell at first as streptozotocin-phosphate.
Links	PubMed
Keywords	Acid Phosphatase; Drug Resistance, Microbial; Escherichia coli; Microbial Sensitivity Tests; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphorylation; Streptozocin
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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: L191 NCBI Taxon ID: 83333	Genotype of Reference Strain: ptsHI Genotype of Experimental Strain : ptsHI	Reference Condition:	OMP:0000276	increased resistance to antibiotics	ECO:0000178	in vivo assay data	Double mutant is resistant to streptozotocin
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